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Universitäts-Herzzentrum Freiburg - Bad KrozingenUniversitäts-Herzzentrum Freiburg - Bad Krozingen

Standard molecular biology methods
  – e.g. Realtime PCR
  – Western blot
  – Cloning

Primary cell isolation and culture
  – Endothelial cells
  – Smooth muscle cells
  – Cell lines
  – Transfection of Plasmids, siRNAs, microRNAs 

Functional cell culture assays
  – Cell proliferation
  – Cell migration
  – Collagen gel contraction

Angiogenesis Assays
  – Matrigel assay
  – 3D collagen spheroid sprouting assay; mosaic spheroids
  – Aortic ring assay
  – Matrigel plug assay 

In vivo models for
  – Angiogenesis
  – Aortic aneurysm
  – Carotis Restenosis
  – PAD

Immunohistochemistry
  – Blood vessel markers, vSMC markers, ECM molecules
  – Proximity Ligation assay (PLA-Assay)
  – Zymography

Contact

PD Dr.rer.nat J. S. Esser
(née Heinke)
Projektleiterin
Principal Investigator

Tel: +49-761-270-70440
e-mail: Jennifer.Esser @universitaets-herzzentrum.de